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Nikon Nis Elements Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 40098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon+nis-elements+software/NIS-Elements/pm42869022-1005-11-11
Average 99 stars, based on 40098 article reviews
Nikon Nis Elements Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 40098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon+nis-elements+software/NIS-Elements/pm42869022-1005-11-11
Average 99 stars, based on 40098 article reviews
nikon nis elements software - by Bioz Stars,
2026-11
99/100 stars
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Software:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Knockdown:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Multiple Displacement Amplification:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Real-time Polymerase Chain Reaction:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Western Blot:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Incubation:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Transfection:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Concentration Assay:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Flow Cytometry:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Cell Culture:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Microscopy:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Northern Blot:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Staining:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Fluorescence In Situ Hybridization:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). Migration:Article Title: <i>Atp13a5</i>Marker Reveals Pericyte Specification in The Mouse Central Nervous System Article Snippet: Research Articles | Cellular/Molecular Atp13a5 Marker Reveals Pericyte Specification in The Mouse Central Nervous System https://doi.org/10.1523/JNEUROSCI.0727-24.2024 Received: 18 April 2024 Revised: 27 August 2024 Accepted: 2 September 2024 Copyright © 2024 Guo et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data. Article Title: Lipid-polymer based complexation and delivery of nucleic acids Article Snippet: After transfection (24 h), media was changed and incubated at 37° C.-5% CO2 atmosphere for another 24 h. qPCR Studies Finally, cells were washed and total RNA was isolated by RNA extraction kit (High Pure RNA Isolation Kit, Roche) according to manufacturer's protocol. Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System Article Snippet: All FISH images presented were projection of Article Title: CD206 + IL-4Rα + MACROPHAGES ARE DRIVERS OF ADVERSE CARDIAC REMODELING IN ISCHEMIC CARDIOMYOPATHY Article Snippet: 4, , , Staining was quantified from more than 5 high-power fields (40x) per section using Article Title: Paracrine Ovarian Cancer Cell-Derived CSF1 Signaling Regulates Macrophage Migration Dynamics in a 3D Microfluidic Model that Recapitulates In Vivo Infiltration Patterns in Patient-Derived Xenografts. Article Snippet: A high density of macrophages in the ovarian cancer microenvironment is associated with disease progression and poor outcomes.. Understanding cancer-macrophage interaction mechanisms that establish this pro-tumorigenic microenvironment is critical for developing macrophage-targeted therapies.. Here, 3D microfluidic assays and patient-derived xenografts are utilized to define the role of cancer-derived colony stimulating factor 1 (CSF1) on macrophage infiltration dynamics toward ovarian cancer cells. Article Title: Vagus nerve stimulation alleviates cardiac dysfunction and inflammatory markers during heart failure in rats. Article Snippet: Vagus nerve stimulation (VNS) is under clinical investigation as a therapy for heart failure with reduced ejection fraction (HFrEF).. This study aimed to investigate its therapeutic effects on three main components of heart failure: cardiac function, cardiac remodeling and central neuroinflammation using a pressure overload (PO) rat model.. Male Sprague-Dawley rats were divided into four groups: PO, PO + VNS, PO + VNS sham, and controls. Article Title: Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption Article Snippet: The cells were permeabilized using 0.2% Triton for 5-10 minutes, washed 3 times with PBS, and incubated with blocking solution (10% donkey serum, 1% BSA, and 0.05% sodium azide) for 30 min. Primary (1:100) and secondary (1:1000) antibodies were diluted in blocking solution and incubated at room temperature for 1 h. Primary antibodies include: anti-RFP (Rockland 200-101-379), anti-FLAG (Sigma-Aldrich F1804), and anti-Myc (Cell Signaling Technology 2278S). |